Circadian Rhythms (2meal Study) Introduction: The 2 meal studies on Circadian Rhythms focus on how rats anticipate two daily meals. The author’s of the study are Mistlberger , Kent, Weinberg, Patton. The study used 16 rats housed in a running wheel cage. The rats were deprived of food for 34 hours and then food was given to them for just one hour twice a day. The time of day that food was given to them was 3:00 and 13:00 hours. Restricted feeding began at 13:00 hours on the 13th day of the study. On the 16th day both meals were restricted from all 16 rats. Along with food being restricted the 16 rats were deprived of light. On day 20 food was given at 6:00 hours. On day 22 the rats were sacrificed and perfused into groups. The results of...The end:
.....the rat both entrain food-anticipatory activity. Physiol Behav . 1994 Jul;56(1):17-25. (originally reviewed for PB in 1993). White W, Timberlake W. Two meals promote entrainment of rat food-anticipatory and rest-activity rhythms. Physiol Behav . 1995 Jun;57(6):1067-74. Mistlberger RE, de Groot MH, Bossert JM, Marchant EG. Discrimination of circadian phase in intact and suprachiasmatic nuclei-ablated rats. Brain Res. 1996 Nov 11;739(1-2):12-8. White W, Timberlake W Meal-engendered circadian-ensuing activity in rats. Physiol Behav . 1999 Jan 1-15;65(4-5):625-42. White W, Schwartz GJ, Moran TH. Meal-synchronized CEA in rats: effects of meal size, intragastric feeding, and subdiaphragmatic vagotomy . Am J Physiol. 1999 May;276(5 Pt 2):R1276-88.